Equipment and Instrumentation

Metabolic Core Main Laboratory

• One Eppendorf EpMotion 5070 liquid handling workstation and one Opentrons OT-2 liquid handling workstation
• VITROS®350 Chemistry System clinical analyzer
• Labconco Centrivap Acid-Resistant with cold trap (-50 °C)
• Bomb calorimeter Parr 6200 and water handling system Parr 6510 (macro and semi-micro operation options)
• FlexiMAP 3D Multiplexing Instrument (with capability for run up to 500 unique assays within a single sample. Immunoassays).
• BioTek Synergy Mx multi-mode plate reader (absorbance, luminescence, fluorescence)
• BioTek ELx405 microplate washer
• AKTA purifier for FPLC with automatic sample fraction collection module
• Bruker LF50 Bentchtop TD-NMR analyzer (mouse body composition)
• Seahorse XFe24 flux analyzer (Agilent)

Mass spectrometry capabilities  For a more detailed list of all analytes routinely measured, see MS-Based Metabolomics

• Nexera X2 UHPLC system coupled to LCMS-8050 (Shimadzu Scientific Instruments). Diana
• Nexera X2 UHPLC system coupled to LCMS-8060 (Shimadzu Scientific Instruments). Athena
• Nexera X2 UHPLC system coupled to LCMS-8060 (Shimadzu Scientific Instruments). Ceres
• Nexera UHPLC system with AI coupled to LCMS-9030 Q-TOF (Shimadzu Scientific Instruments). Cybele
• CLAM2030 fully automated sample preparation module coupled to Nexera X2 UHPLC system and to LCMS-8060 (Shimadzu Scientific Instruments). Hera
• Nexera UC Supercritical Fluid Chromatograph coupled to LCMS-8060 (Shimadzu Scientific Instruments). Aphrodite

Animal Facility

• Urine collection chambers for rodents (8 cages)
• Echo MRI-100 analyzer (mouse body composition)
• TSE Labmaster indirect calorimetry system (24 cages)
• TSE Phenomaster climate-controlled indirect calorimetry system (36 cage)
• Columbus Instruments rodent treadmills (18 lanes)
• Stellar Implantable Transmitters coupled to indirect calorimetry (12 cages)
• Bruker SKYSCAN 1276. High-resolution, fast in-vivo desktop micro-CT scanner

Metabolic Phenotyping Core

For Service requests please go to the ILab portal

 Main lab location L4.102

Contact:

                         For chemistries and LC/MS/MS services contact ruth.gordillo@utsouthwestern.edu

For rodent metabolic chambers contact Teppei.Fujikawa@UTSouthwestern.edu

Our goal is to expand the scope of techniques available to investigators, standardize key methodologies, and expedite the completion of research on diseases related to metabolic disorders (diabetes and obesity), cancer, aging neurological disorders, etc.

Preferred way of contact is Email. We provide letters of support for grant submissions both on and off campus. The Metabolic Phenotyping Core (MPC) provides state-of-the-art analytical and phenotypical measures to the scientific community both inside and outside UT Southwestern Medical Center.