Mass Spectrometry-based Metabolomics Portfolio

Contact core director for instructions for sample collection and submission.

Ruth.gordillo@utsouthwestern.edu

Routine analysis.

1. Sphingolipids Profiling (mammalian tissues): LC-ESI-MS/MS. All profiled lipids with sphingoid base backbone C18:1 or C18:0

https://doi.org/10.1186/1476-511X-9-84

 Sphingoid Bases: Sphingosine, Sphinganine, Deoxysphingosine, DeoxySphinganine, Sphingosine1P, Sphingaine1P

Ceramides: Ceramide C14:0, Ceramide C16:0, Ceramide C18:1, Ceramide C18:0, Ceramide C20:0, Ceramide C22:0, Ceramide C24:1, Ceramide C24:0, Ceramide C26:1, Ceramide C26:0.

DihydroDihydroCeramides: DihydroCeramide C14:0, DihydroCeramide C16:0, DihydroCeramide C18:0, DihydroCeramide C20:0, DihydroCeramide C22:0, DihydroCeramide C24:1, DihydroCeramide C24:0,

HexosylCeramides: HexosylCeramide C14:0, HexosylCeramide C16:0, HexosylCeramide C18:0, HexosylCeramide C20:0, HexosylCeramide C22:0, HexosylCeramide C24:1, HexosylCeramide C24:0.

LactosylCeramides: LactosylCeramide C14:0, LactosylCeramide C16:0, LactosylCeramide C18:0, LactosylCeramide C20:0, LactosylCeramide C22:0, LactosylCeramide C24:1, LactosylCeramide C24:0.

Sphingomyelins:

SPHINGOMYELIN14:0, SPHINGOMYELIN14:1, SPHINGOMYELIN16:1, SPHINGOMYELIN16:0, SPHINGOMYELIN18:1, SPHINGOMYELIN18:2, SPHINGOMYELIN18:3, SPHINGOMYELIN18:0, SPHINGOMYELIN20:0, SPHINGOMYELIN20:1, SPHINGOMYELIN20:2, SPHINGOMYELIN20:3, SPHINGOMYELIN20:4, SPHINGOMYELIN20:5, SPHINGOMYELIN22:1, SPHINGOMYELIN22:0, SPHINGOMYELIN22:6, SPHINGOMYELIN24:1, SPHINGOMYELIN24:0.

2. Diastereomeric separation of Glucosyl-Ceramides and Galactosyl-Ceramides (mammalian tissues): SFC-ESI-MS/MS. All profiled lipids with sphingoid base backbone C18:1. Semiquantitative 

https://doi.org/10.1016/j.biopha.2021.111565

3. Bile Acids profiling (mammalian tissues): LC-ESI-MS/MS.

List of Bile Acids targeted in this analysis: TaMCA, TomegaMCA, TbMCA, TUDCA, GUDCA, THDCA, GHDCA, oMCA, aMCA, 7keto-DCA, TCA, bMCA, GCA, UDCA, HDCA, CA, GCDCA, 7keto-LCA, 2keto-LCA, GDCA, TCDCA, TDCA, CDCA, allo-CDCA, DCA, GLCA, LCA, allo-LCA, TLCA.

ASMS2021 Philadelphia ThP188

4. Free Amino Acids profiling (mammalian tissues): LC-ESI-MS/MS.

5. Cell Culture Supertnatant Metabolite Profiling (mammalian tissues): LC-ESI-MS/MS. Semiquantitative

The method targets culture medium components and metabolites secreted by cells. This culture medium analysis platform enables the simultaneous analysis of up to 95 components. Touchstone Diabetes Center is using this methodology to monitor cell differentiation and cell culture response to treatment.

ASMS2021 Philadelphia ThP189

6. Lysophosphatidic Acids and Phosphatidic Acids profiling. (mammalian tissues): SFC-ESI-MS/MS. Semiquantitative

To learn more about phosphatidic acids and lysophosphatidic acids see links below:

https://www.lipidmaps.org/resources/lipidweb/lipidweb_html/lipids/complex/pa/index.htm

https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/phosphatidic-acid

https://febs.onlinelibrary.wiley.com/doi/10.1002/1873-3468.13563

https://doi.org/10.1016/j.bbalip.2009.04.001

7.Phospholipids profiling. (mammalian tissues): LC-ESI-MS/MS. Semiquantitative

Lysophosphatidylcholines, Phosphatidylcholines, Lysophosphatidylethanolamines, Phosphatidylethanolamines, Lysophosphatidylinositols, Phosphatidylinositols, Lysophosphatidylserine, Phosphatidylserines, Lysophosphatidylglycerols, Phosphatidylglycerols.

References:

https://doi.org/10.1016/j.jbc.2021.101470

10.1016/j.cmet.2021.06.001

https://doi.org/10.1038/s42255-024-01078-9

Notes:

Some of the listed metabolites might not be detected in certain tissues.

Check our publications on the following link. Contact the core director if interested in other mass spectrometry-based assays described in our articles.